Sören Starck: Microbial production of cis,cis-muconic acid from hydrothermally converted lignocellulose
Microbial production of cis,cis-muconic acid from hydrothermally converted lignocellulose
Buch
- Jentzsch-Cuvillier, Annette, 03/2022
- Binding: Kartoniert / Broschiert, Paperback
- Language: Englisch
- ISBN-13: 9783736975897
- Order number: 10964364
- Volume: 134 Pages
- Weight: 184 g
- Format: 210 x 148 mm
- Thickness: 7 mm
- Release date: 17.3.2022
- Series: Hochschulschriften - Institut für Systembiotechnologie, Universität des Saarlandes 8
Caution: Product is not in German language
Blurb
Cis, cis-muconic acid receives increasing interest to be produced from renewables. Catabolic microbial pathways can be tailored to accumulate cis, cis-muconic acid from a range of aromatic compounds. A renewable, sustainable and under-valued resource for aromatics is lignin. In this work, using hydrothermal conversion, lignin was depolymerized into hydrolysates with up to 615 mM aromatic monomer content. Catechol-rich hydrolysates were generated for bioconversion with the previously developed cis, cis-muconic acid producers P. putida MA-9 and C. glutamicum MA-2, whereas hydrolysates were guaiacol-rich for Amycolatopsis sp. MA-2. When grown with glucose as a co-substrate, C. glutamicum MA-2 yielded 2.6 g L ¹ (100 % yield) cis, cis-muconic acid from catechol. Towards an even more sustainable process, glucose was then replaced by hemicellulose, a non-food renewable. Hemicellulose, a co-constituent of lignin in lignocellulose, was hydrothermally converted into a mixture of C and C sugars. As hemicellulose was mainly converted into xylose (91 % yield), C. glutamicum MA-2 was engineered to utilize this pentose. Fed-batch bioconversion on a catechol-rich Kraft lignin hydrolysate as well as a hemicellulose hydrolysate using C. glutamicum MA-4 yielded 4 g L ¹ muconic acids. As the developed process was non-competitive to feed and food, it is a promising starting point for future application in bio-based industrial settings.Notes:
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