Kurzbeschreibung
Dieses Kursmanual beschreibt genetische Experimente an der Fruchtfliege Drosophila, die ohne aufwendige Ausrüstung in jedem Labor durchgeführt werden können. Es ist damit für Studentenpraktika ideal geeignet und ebenso für ein schnelles Screening auf Toxizität und Mutagenizität von neu entwickelten Substanzen.
Inhaltsangabe
1. General.- 1.1 Why Drosophila?.- 1.2 Basic Equipment.- 1.3 Genetic Terminology.- 1.4 The Biology of Drosophila melanoqaster.- 1.5 Methods for Culturing and Studying Drosophila melanoqaster.- 1.6 List of Genetic Symbols and Stocklist.- 1.7 General Books on Drosophila.- 2. Morphology of Drosophila Melanogaster.- 2.1 Eggs and Embryos.- 2.2 L3 Larvae.- 2.3 Pupae.- 2.4 Adult Flies.- 2.5 Some Mutants.- 3. Transmission Genetics.- 3.1 Dihybrid Cross with Independent Assortment.- 3.2 Sex-Linked Inheritance.- 3.3 Dihybrid Test Cross with Linked Genes.- 3.4 Sex Determination.- 3.5 Genetic Localization of Mutations Within the Genome.- 3.6 Mapping on a Chromosome.- 3.7 Segregation of Compound Chromosomes.- 3.8 Meiotic Mutants.- 4. Phenogenetics.- 4.1 Temperature Effect on Expression of Phenotype.- 4.2 Temperature Effect on a Homeotic Mutant.- 4.3 Mutants with Abnormal Eye Color.- 4.4 Chromatographic Analysis of Eye Color Mutants.- 4.5 Transplantation of Eye Imaginai Discs.- 4.6 Supplementation of an Eye Color Mutant.- 4.7 Genetic Complementation and Allelism.- 4.8 Transposable Elements.- 5. Mutation Genetics.- 5.1 Induction and Detection of Sex-Linked Recessive Lethals.- 5.2 Induction and Detection of Reciprocal Translocations.- 5.3 Mutagen-Induced Sex Chromosome Losses.- 5.4 Somatic Mutation and Mitotic Recombination.- 6. Population Genetics.- 6.1 Determination of Allele Frequencies in Populations in Hardy-Weinberg Equilibrium.- 6.2 Variations in Populations with Natural and Artificial Selection.- 6.3 Mating Behavior.- 6.4 Mating Preferences.- 7. Cytology and Cytogenetics.- 7.1 Microscopic Analysis of Mitotic and Polytene Chromosomes.- 7.2 Balancer Chromosomes.- 8. Molecular Biology of Drosophila: An Overview.- 9. Results and Answers.- 10. Subject Index.
Klappentext
The Biological Sciences are in the midst of a scientific rev olution. During the past decade under the rubric of molecu lar biology, chemistry and physics have assumed an integral role in biological research. This is especially true in ge netics, where the cloning of genes and the manipulation of genomic DNA have become in many organisms routine laboratory procedures. These noteworthy advances, it must be empha sized, especially in molecular genetics, are not autonomous. Rather, they have been accomplished with those organisms whose formal genetics has been documented in great detail. For the beginning student or the established investigator who is interested in pursuing eukaryote molecular genetic re search, Drosophila melanogaster, with its rich body of formal genetic information is one organism of choice. The book "Drosophila Genetics. A Practical Course" is an indispens able source of information for the beginner in the biology and formal genetics of Drosophila melanogaster. The scope of this guide, a revision and enlargement of the original German language version, is broad and instructive. The information included ranges from the simple, but necessary, details on how to culture and manipulate Drosophila flies to a series of more sophisticated genetic experiments. After completing the experiments detailed in the text, all students - neophyte or experienced - will be richly rewarded by having acquired a broad base of classical genetics information relevant for the biologist in its own right and prerequisite to Drosophila genetics research - formal and / or molecular. Davis, California, Melvin M.
