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This book presents cutting-edge research and developments in the field of medical and biological engineering, which a special emphasis on activities carried out in the Asian-Pacific region. Gathering the proceedings of the 12th Asian-Pacific Conference on Medical and Biological Engineering (APCMBE 2023), held on May 18 21, 2023, in Suzhou, China, this second volume of a two-volume set covers advances in computer-aided surgery, biomechanics and micro-nanoengineering, health informatics and health engineering, as well as computational modeling and simulation, as well as AI applications in biology and medicine. It addresses a broad audience of researchers and professionals active in biomedical engineering, biomechanics, medical biophysics, and health informatics.